A Proteomics Approach for the Identification of DNA Binding Activities Observed in the Electrophoretic Mobility Shift Assay
نویسندگان
چکیده
منابع مشابه
A proteomics approach for the identification of DNA binding activities observed in the electrophoretic mobility shift assay.
Transcription factors lie at the center of gene regulation, and their identification is crucial to the understanding of transcription and gene expression. Traditionally, the isolation and identification of transcription factors has been a long and laborious task. We present here a novel method for the identification of DNA-binding proteins seen in electrophoretic mobility shift assay (EMSA) usi...
متن کاملElectrophoretic Mobility Shift Assay (EMSA).
Transcriptional regulation of gene expression is controlled through the binding of sequence-specific DNA-binding proteins (transcription factors) to the regulatory regions of genes. The exact gene expression program of a cell is determined by the spectrum of transcription factors present with the nucleus of a cell. The presence of these factors is dependent upon the cell type being examined and...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2002
ISSN: 1535-9476
DOI: 10.1074/mcp.t200003-mcp200